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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 818

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
2,4-Dichlorophenoxyacetic acid	Not Given		0.0041	Acetone	Guinea pig	Back	4.2	2	24	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
48	101	3.85	60.9				1.3	0.89		38.8	10.59	38.8

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					4

NOTES

Hairless guinea pigs were used. The dose site was protected from surface rub off by a foam rubber non-occlusive patch and washed at 24 hours with radiac soap solution. The amount left in the skin was measured by LSC analysis of digests of skin taken at autopsy 14 days after exposure.

REFERENCE

In vitro Dermal Absorption of Pesticides: V. In Vivo and In Vitro Comparison of the Herbicide 2,4-Dichlorophenoxyacetic Acid in Rat, Guinea Pig, Pig, Human and Tissue-cultured skin (1994) Toxicol. Vitro, 8 :1219-1224