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In Vivo Study No: 838

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
Caffeine0.029.710.1942ethyleneglycol/H2O/tritonx100 (90/10)/[H2O] (50/50)HumanForehead120.5Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
24        0.004342.2350.2392.235
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
     7  

NOTES

Caucasian Males were used. Results were given in nmols. I have converted these to grams for this database. After 30 minutes the excess solution at the application site was washed off using ethanol-wate mixture (95:5) followed by two rinses with distilled water. The application site was then dried with cotton buds. Amount absorbed was calculated by the amount excreted in the urine, corrected for incomplete excretion.

REFERENCE

In Vivo Relationship Between Transepidermal Water Loss and Percutaneous Penetration of Some Organic Compounds in Man: Effect of Anatomic Site (1987) Arch. Dermatol. Res., 279 :351-356