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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 838

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Caffeine	0.02	9.71	0.1942	ethyleneglycol/H2O/tritonx100 (90/10)/[H2O] (50/50)	Human	Forehead	1	2	0.5	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
24									0.00434	2.235	0.239	2.235

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

0.00434

2.235

0.239

2.235

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					7

NOTES

Caucasian Males were used. Results were given in nmols. I have converted these to grams for this database. After 30 minutes the excess solution at the application site was washed off using ethanol-wate mixture (95:5) followed by two rinses with distilled water. The application site was then dried with cotton buds. Amount absorbed was calculated by the amount excreted in the urine, corrected for incomplete excretion.

REFERENCE

In Vivo Relationship Between Transepidermal Water Loss and Percutaneous Penetration of Some Organic Compounds in Man: Effect of Anatomic Site (1987) Arch. Dermatol. Res., 279 :351-356