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In Vivo Study No: 843

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
n-Methyl-2-pyrrolidone0.2103320.66None (Neat)Ratback1020.25Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
0.2596.81.08160.7377.8  27.0613.112.39660.386
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
 4958.4154.95  4  

NOTES

A circular ring was glued to the back of the rats to mark the application area. Fter application the site was covered with a perforated circular plastic cup to allow aeration. Results were given +/- SEM. I have converted this to SD for the purpose of this database. The % absoprtion is calculated from the % in the urine + carcass + collected blood + washing cage. They got a plasma concentration of 0.045 %/g

REFERENCE

Toxicokinetics and Metabolism of N-[14C]N-Methyl-2-Pyrrolidone in Male Sprague-Dawley Rats: In Vivo and In Vitro Percutaneous Absorption (2003) Drug Met. Dispos., 31 :659-669