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In Vivo Study No: 844

METHODS

ChemicalMembraneConcentration mg/mlConcentration mg/mlloading (mg/cm2)SpeciesSiteArea (cm2)Occluded?Exposure Time (h)Analytical Method
n-Methyl-2-pyrrolidone0.2103320.66None (Neat)Ratback1020.5Radiolabelled (14C)

RESULTS

Length of Study (h)% Recovery+/-Dose remaining on surface (mg)% remaining on SurfaceDose remaining in stratum corneum (mg)% remaining in stratum corneumDose remaining in the viable skin% remaining in the viable skinAmount Absorbed (mg)% Absorbed+/-% Absorbed
0.597.467.18126.4461.2  35.4717.1737.2518.035.518.03
Maximal Flux (mcrg/cm2/h)Average Flux (mcrg/cm2/h)+/-Time Plasma/Blood Levels Peaked (h)Peak Blood Concentration (mg/l)nkp (cm/h)Lag Time (h)
 7447.931136.3  4  

NOTES

A circular ring was glued to the back of the rats to mark the application area. Fter application the site was covered with a perforated circular plastic cup to allow aeration. Results were given +/- SEM. I have converted this to SD for the purpose of this database. The % absoprtion is calculated from the % in the urine + carcass + collected blood + washing cage. They got a plasma concentration of 0.096 %/g

REFERENCE

Toxicokinetics and Metabolism of N-[14C]N-Methyl-2-Pyrrolidone in Male Sprague-Dawley Rats: In Vivo and In Vitro Percutaneous Absorption (2003) Drug Met. Dispos., 31 :659-669