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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 845

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
n-Methyl-2-pyrrolidone	0.2	1033	20.66	None (Neat)	Rat	back	10	2	0.75	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
0.75	93.43	2.66	105.53	51.08			28.3	13.7	59.19	28.65	2.77	28.65

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
	7892.12	764.42			4

NOTES

A circular ring was glued to the back of the rats to mark the application area. Fter application the site was covered with a perforated circular plastic cup to allow aeration. Results were given +/- SEM. I have converted this to SD for the purpose of this database. The % absoprtion is calculated from the % in the urine + carcass + collected blood + washing cage. They got a plasma concentration of 0.17 %/g

REFERENCE

Toxicokinetics and Metabolism of N-[14C]N-Methyl-2-Pyrrolidone in Male Sprague-Dawley Rats: In Vivo and In Vitro Percutaneous Absorption (2003) Drug Met. Dispos., 31 :659-669