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IN VIVO STUDIES FURTHER DETAILS

In Vivo Study No: 858

METHODS

Chemical	Membrane	Concentration mg/ml	Concentration mg/ml	loading (mg/cm2)	Species	Site	Area (cm2)	Occluded?	Exposure Time (h)	Analytical Method
Alachlor	0.18	2.22	0.01	ME (17:1 water dilution) : LASSO	Monkey	abdomen	40	2	12	Radiolabelled (14C)

RESULTS

Length of Study (h)	% Recovery	+/-	Dose remaining on surface (mg)	% remaining on Surface	Dose remaining in stratum corneum (mg)	% remaining in stratum corneum	Dose remaining in the viable skin	% remaining in the viable skin	Amount Absorbed (mg)	% Absorbed	+/-	% Absorbed
	89.7		0.339	84.73					0.02	4.97		4.97

Length of Study (h)

% Recovery

+/-

Dose remaining on surface (mg)

% remaining on Surface

Dose remaining in stratum corneum (mg)

% remaining in stratum corneum

Dose remaining in the viable skin

% remaining in the viable skin

Amount Absorbed (mg)

% Absorbed

+/-

% Absorbed

89.7

0.339

84.73

0.02

4.97

Maximal Flux (mcrg/cm2/h)	Average Flux (mcrg/cm2/h)	+/-	Time Plasma/Blood Levels Peaked (h)	Peak Blood Concentration (mg/l)	n	kp (cm/h)	Lag Time (h)
					3

NOTES

Male and female young adult rhesus monkeys were used. The monkeys were kept in restraining chairs for the duration of the experiment and a teflon drape was placed around the abdomen of each animal below the application site to collect and drips of test material or expoliated skin. The application site had been lightly clipped 48 hours prior to exposure. After 12 hours the application sites were swabbed with gauze pads wetted with a 10% solution of ivory soap. % absorption was calculated from the amounts recovered in the urine + faeces + tissues and carcass. Only 2 animals were sacrificed. These results are only for 3 monkeys as one was an outlier to the others

REFERENCE

Percutaneous Absorption and Excretion of Alachlor in Rhesus Monkeys (1988) Fundam. Appl. Toxicol., 10 :664-671